| 质粒名称: | lentiCRISPR v2-sgSLC7A11/xCT-1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 161818 |
| 存储实验室: | Boyi Gan |
|
载体骨架基本信息 |
|
| 载体名称: | lentiCRISPR v2 |
| 载体出品公司: | Feng Zhang |
| 空载体大小: | 12000 bp |
| 完整质粒大小: | 12000 bp |
| 载体类型: | 慢病毒，CRISPR |
| 筛选标记: | 嘌呤霉素 |
| 载体抗性: | 氨苄霉素, 100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: |
NEB Stable
|
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | SLC7A11 solute carrier family 7 member 11 xCT |
| 物种来源: | H. sapiens (human) |
| GenBank编号 | NM_014331.4 |
|
文献引用方法 |
|
| 材料和方法部分: | lentiCRISPR v2-sgSLC7A11/xCT-1 was a gift from Boyi Gan (Addgene plasmid # 161818 ; http://n2t.net/addgene:161818 ; RRID:Addgene_161818) |
| 参考文献部分: | Cystine transporter regulation of pentose phosphate pathway dependency and disulfide stress exposes a targetable metabolic vulnerability in cancer. Liu X, Olszewski K, Zhang Y, Lim EW, Shi J, Zhang X, Zhang J, Lee H, Koppula P, Lei G, Zhuang L, You MJ, Fang B, Li W, Metallo CM, Poyurovsky MV, Gan B. Nat Cell Biol. 2020 Apr;22(4):476-486. doi: 10.1038/s41556-020-0496-x. Epub 2020 Mar 30. 10.1038/s41556-020-0496-x PubMed 32231310 |
买家导航
lentiCRISPR v2-sgSLC7A11/xCT-1载体质粒基本信息
lentiCRISPR v2-sgSLC7A11/xCT-1质粒图谱载体图谱和lentiCRISPR v2-sgSLC7A11/xCT-1载体序列质粒序列多克隆位点信息
lentiCRISPR v2-sgSLC7A11/xCT-1质粒载体简介
lentiCRISPR v2-sgSLC7A11/xCT-1质粒序列载体序列
LOCUS lentiCRISPR v2-sgSLC7A11/xCT-1 13013 bp DNA circular SYN 15-DEC-2025
DEFINITION knock out SLC7A11/xCT in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13013)
AUTHORS Liu X, Olszewski K, Zhang Y, Lim EW, Shi J, Zhang X, Zhang J, Lee H,
Koppula P, Lei G, Zhuang L, You MJ, Fang B, Li W, Metallo CM,
Poyurovsky MV, Gan B
TITLE Cystine transporter regulation of pentose phosphate pathway
dependency and disulfide stress exposes a targetable metabolic
vulnerability in cancer.
JOURNAL Nat Cell Biol. 2020 Apr;22(4):476-486. doi:
10.1038/s41556-020-0496-x. Epub 2020 Mar 30.
PUBMED 32231310
REFERENCE 2 (bases 1 to 13013)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/s41556-020-0496-x"; journalName: "Nat Cell Biol"; date:
"2020-04"; volume: "22"; issue: "4"; pages: "476-486"
COMMENT Sequence Label: lentiCRISPR v2-sgSLC7A11/xCT-1
FEATURES Location/Qualifiers
source 1..13013
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 382..580
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 598..778
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 825..950
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1443..1676
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1861..1905
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2203..2320
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2371..2619
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA
(Domitrovich & Kunkel, 2003)"
primer_bind 2371..2391
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 2542..2561
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 2641..2716
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 2778..2989
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
regulatory 3008..3017
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3014..7117
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 3014..7117
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 7118..7165
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 7166..7189
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 7199..7255
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 7256..7852
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind 7749..7769
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 7868..8456
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7921..7941)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8339..8350)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 8528..8761
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(8787..8804)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 8793..9017
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 9063..9491
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9150..9169)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9360..9381
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(9500..9520)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9505..9834
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9685..9820
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9747..9766
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 9882..9929
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 9948..10322
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 10452..10573
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10489..10508)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10543..10562
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(10622..10638)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10622..10638)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10635..10657)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10646..10662
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(10670..10676,10677..10694,10695..10700))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10715..10736
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(10853..10870)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11024..11612)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11104..11123)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(11783..12574,12575..12643))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12406..12425
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12644..12748)
/gene="bla"
/label=AmpR promoter
primer_bind complement(12823..12842)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
2341 cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat tccttcatat
2401 ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca
2461 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
2521 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
2581 ttcttggctt tatatatctt gtggaaagga cgaaacaccg atgagcttga tcgcaagttc
2641 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
2701 ggcaccgagt cggtgctttt ttgaattcgc tagctaggtc ttgaaaggag tgggaattgg
2761 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
2821 aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
2881 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
2941 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg accggttcta
3001 gagcgctgcc accatggaca agaagtacag catcggcctg gacatcggca ccaactctgt
3061 gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg
3121 caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg
3181 cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa
3241 gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag
3301 cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca
3361 ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta
3421 ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct
3481 ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc
3541 cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt
3601 cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact
3661 gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg
3721 cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt
3781 cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga
3841 caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct
3901 gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc
3961 cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa
4021 agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa
4081 gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat
4141 caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga
4201 ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct
4261 gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa
4321 ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc
4381 caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg
4441 gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac
4501 caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga
4561 gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa
4621 gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa
4681 ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga
4741 ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga
4801 tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct
4861 ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct
4921 gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata
4981 caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg
5041 caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct
5101 gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca
5161 gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg
5221 catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc
5281 cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa
5341 cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct
5401 gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct
5461 gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta
5521 cgatgtggac catatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt
5581 gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt
5641 gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa
5701 gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt
5761 catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga
5821 ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat
5881 caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg
5941 cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc
6001 cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta
6061 cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta
6121 cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga
6181 gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa
6241 gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa
6301 aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag
6361 cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag
6421 ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa
6481 actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa
6541 gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat
6601 caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc
6661 tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct
6721 gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca
6781 gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
6841 ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa
6901 gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac
6961 caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta
7021 caccagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta
7081 cgagacacgg atcgacctgt ctcagctggg aggcgacaag cgacctgccg ccacaaagaa
7141 ggctggacag gctaagaaga agaaagatta caaagacgat gacgataagg gatccggcgc
7201 aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgaccga
7261 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
7321 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
7381 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
7441 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
7501 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
7561 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
7621 gttcctggcc accgtcggag tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
7681 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
7741 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
7801 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgtta
7861 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
7921 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
7981 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
8041 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
8101 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
8161 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
8221 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
8281 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
8341 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
8401 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
8461 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
8521 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
8581 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
8641 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
8701 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
8761 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
8821 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
8881 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
8941 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
9001 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
9061 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
9121 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
9181 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
9241 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
9301 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
9361 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
9421 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
9481 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
9541 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
9601 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
9661 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
9721 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
9781 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
9841 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
9901 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
9961 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
10021 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
10081 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
10141 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
10201 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
10261 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
10321 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
10381 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
10441 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
10501 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
10561 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
10621 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
10681 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
10741 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
10801 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
10861 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
10921 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
10981 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
11041 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
11101 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
11161 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
11221 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
11281 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
11341 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
11401 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
11461 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
11521 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
11581 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
11641 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
11701 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
11761 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
11821 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
11881 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
11941 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
12001 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
12061 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
12121 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
12181 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
12241 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
12301 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
12361 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
12421 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
12481 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
12541 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
12601 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
12661 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
12721 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
12781 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
12841 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
12901 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
12961 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gtt
//
lentiCRISPR v2-sgSLC7A11/xCT-1载体图谱质粒图谱pdf版和lentiCRISPR v2-sgSLC7A11/xCT-1载体序列质粒序列等相关资料下载
lentiCRISPR v2-sgSLC7A11/xCT-1质粒载体应用举例
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